Composite

Part:BBa_K3002219

Designed by: Dorothée Klein   Group: iGEM19_TU_Kaiserslautern   (2019-10-21)
Revision as of 01:47, 22 October 2019 by MSchlosser (Talk | contribs)


L2 spectinomycin resistance + ARS_Mut-PETase_SP20HA

This composite part contains a spectinomycin resistance (BBa_K3002102) and the mutant PETase with the secretion signal ARS (BBa_K3002111) fused with an SP20HA-tag for easy detection via HA-antibody and enhanced secretion.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2401
    Illegal PstI site found at 3491
    Illegal PstI site found at 4464
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2401
    Illegal NheI site found at 2665
    Illegal PstI site found at 3491
    Illegal PstI site found at 4464
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2401
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2401
    Illegal PstI site found at 3491
    Illegal PstI site found at 4464
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2401
    Illegal PstI site found at 3491
    Illegal PstI site found at 4464
    Illegal NgoMIV site found at 1401
    Illegal NgoMIV site found at 1584
    Illegal NgoMIV site found at 1694
    Illegal NgoMIV site found at 3226
    Illegal NgoMIV site found at 3253
    Illegal NgoMIV site found at 5024
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None