Composite

Part:BBa_K3002216

Designed by: Dorothée Klein   Group: iGEM19_TU_Kaiserslautern   (2019-10-21)
Revision as of 01:44, 22 October 2019 by MSchlosser (Talk | contribs)


L2 spectinomycin resistance + L1_connector_Pos2 + ccA_MHETase

This composite part contains a spectinomycin resistance (BBa_K3002102) and the MHETase with the cCA (BBa_K3002113) fused with an HA-tag for easy detection via HA-antibody.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 3738
    Illegal PstI site found at 4062
    Illegal PstI site found at 4405
    Illegal PstI site found at 5215
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2660
    Illegal PstI site found at 3738
    Illegal PstI site found at 4062
    Illegal PstI site found at 4405
    Illegal PstI site found at 5215
    Illegal NotI site found at 4073
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4983
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 3738
    Illegal PstI site found at 4062
    Illegal PstI site found at 4405
    Illegal PstI site found at 5215
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 3738
    Illegal PstI site found at 4062
    Illegal PstI site found at 4405
    Illegal PstI site found at 5215
    Illegal NgoMIV site found at 1401
    Illegal NgoMIV site found at 1584
    Illegal NgoMIV site found at 1694
    Illegal NgoMIV site found at 3671
    Illegal NgoMIV site found at 4132
    Illegal NgoMIV site found at 4162
    Illegal NgoMIV site found at 4477
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None