Composite

Part:BBa_K3002213

Designed by: Dorothée Klein   Group: iGEM19_TU_Kaiserslautern   (2019-10-21)
Revision as of 01:39, 22 October 2019 by MSchlosser (Talk | contribs)


L2 spectinomycin resistance + ARS_Mut-PETase_SP20HA + cCA_MHETase_SP20HA

This composite part contains a spectinomycin resistance (BBa_K3002102), the mutant PETase with the secretion signal ARS (BBa_K3002111) and the MHETase with the cCA (BBa_K3002114), both fused with an SP20HA-tag for easy detection via HA-antibody and enhanced secretion.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5423
    Illegal PstI site found at 3491
    Illegal PstI site found at 4464
    Illegal PstI site found at 6765
    Illegal PstI site found at 7089
    Illegal PstI site found at 7432
    Illegal PstI site found at 8242
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5423
    Illegal NheI site found at 2665
    Illegal NheI site found at 5687
    Illegal PstI site found at 3491
    Illegal PstI site found at 4464
    Illegal PstI site found at 6765
    Illegal PstI site found at 7089
    Illegal PstI site found at 7432
    Illegal PstI site found at 8242
    Illegal NotI site found at 7100
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5423
    Illegal BglII site found at 8010
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5423
    Illegal PstI site found at 3491
    Illegal PstI site found at 4464
    Illegal PstI site found at 6765
    Illegal PstI site found at 7089
    Illegal PstI site found at 7432
    Illegal PstI site found at 8242
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5423
    Illegal PstI site found at 3491
    Illegal PstI site found at 4464
    Illegal PstI site found at 6765
    Illegal PstI site found at 7089
    Illegal PstI site found at 7432
    Illegal PstI site found at 8242
    Illegal NgoMIV site found at 1401
    Illegal NgoMIV site found at 1584
    Illegal NgoMIV site found at 1694
    Illegal NgoMIV site found at 3226
    Illegal NgoMIV site found at 3253
    Illegal NgoMIV site found at 5024
    Illegal NgoMIV site found at 6698
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None