Regulatory

Part:BBa_K2992040

Designed by: Yaseen Tengur   Group: iGEM19_Nottingham   (2019-10-01)
Revision as of 01:28, 22 October 2019 by JacobGausden (Talk | contribs) (→‎Characterisation)


Pha33 from C. botulinum

Promoter region from the hemagglutinin component of botulinum neurotoxin gene cluster.

Usge and Biology

This parts entry represents the promoter region for the hemagglutinin component of the botulinum neurotoxin complex from C. botulinum. Since ha33 is natural constituent of the BotR regulon, we chose this promoter to control the expression of our FAST reporter construct thus linking reporter fluorescence with botulinum neurotoxin production. In doing so, we hoped to generate our surrogate host strain as a model for predicting neurotoxin production in foodstuffs following food manufacturing processes.


Characterisation

Data incoming. FAST.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Dupuy, B. et al., 2006. Regulation of toxin and bacteriocin gene expression in Clostridium by interchangeable RNA polymerase sigma factors. Molecular Microbiology, 60(4), pp.1044–1057.


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