Part:BBa_K2970001

Trigger 2

Trigger 2 forms together with Trigger 1 (BBa_K2970000) a complex due to complementary regions, as shown in Figure 1A. This complex can open a gate (BBa_K2970002, Figure 1B) to enable translation of a gene that is locked with this gate to regulate translation. After binding of the trigger complex due to complementarity, the hairpin of the gate will open and release the ribosome binding site and the start codon, thus translation can be initiated (Figure 2). The affinity between the trigger complex and the gate is greater than that of the gate to itself (in the hairpin). A single trigger cannot open the gate because it only contains half the required complementary sequence.

Figure 1: A) Formation of trigger complex after translation. B) mRNA of gate sequence forms secondary structures that hide the ribosome binding site and the start codon.

Figure 2: Opening of the gate due to annealing of trigger complex to gate.

To transform the trigger into bacteria it should be put on a plasmid together with a promoter and a terminator as we did (BBa_K2970004). After transformation of both trigger plasmids and the gate plasmid in one bacterium all three mRNA structures will be formed, the gate opens, and the translation of the gene of interest can be initiated.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]