Composite

Part:BBa_K3262002

Designed by: Shrihari Negi   Group: iGEM19_SNU_India   (2019-10-16)
Revision as of 01:24, 22 October 2019 by Aakash Grover (Talk | contribs) (→‎Overlap Extension PCR to develop construct (2019 SNU_India))


J23115+Estrogen-SensitiveT7RNAP

Estrogen sensitive T7 RNA polymerase wit constitutive promoter

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 2577
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 819
    Illegal XhoI site found at 800
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2315
    Illegal AgeI site found at 1418
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1145
    Illegal BsaI.rc site found at 2606
    Illegal SapI.rc site found at 560


Overlap Extension PCR to develop construct (2019 SNU_India)

Figure 1:Agarose gel electrophoresis of IDT gBlocks suspended in milliQ and loaded on 1% agarose gel.
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