Composite

Part:BBa_K3156888

Designed by: Tingzhen Liu   Group: iGEM19_SHSBNU_China   (2019-10-16)
Revision as of 01:23, 22 October 2019 by Marshalliu (Talk | contribs) (→‎Usage and Biology)


pLac Promoter-ssDNA[sfGFP(ON)]-Ec86-Beta

Design

We combined msd-msr cassette, Reverse Transcriptase Ec-86 and Beta recombinase together to create BBa_K3156888. Msd-msr cassette can produce mRNA for reverse transcription to produce

Figure 1.Circuit design of BBa_K3156888(sfgfp forward).

We created a

Figure 2.Stimulated reparing progress of K3156888.

Usage and Biology

Figure 1.Repairing result of BBa_K3156888(sfgfp forward).

Figure 1.Repairing result of BBa_K3156888(sfgfp reverse).

Reference

[1]F. Farzadfard, T. K. Lu, Science 346,1256272 (2014). DOI: 10.1126/science.1256272

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 9
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1
    Illegal XhoI site found at 516
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/composite_part/nominee
Parameters
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