Composite

Part:BBa_K3002127

Designed by: Dorothée Klein   Group: iGEM19_TU_Kaiserslautern   (2019-10-16)
Revision as of 01:22, 22 October 2019 by MSchlosser (Talk | contribs)


Level 1 – Hygromycin Resistance Gene for Chlamydomonas reinhardtii (Phytobrick)

This composite part contains the PSAD-promoter (BBa_K3002001) in combination with the PSAD-Terminator (BBa_K3002002) and the coding sequence of the Hygromycin resistance (BBa_K3002013) plus the MoClo connectors for positions B2-B3 (BBa_K3002303) and B5-B6 (BBa_K3002305). This part mediates resistance to hygromycin.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1270
    Illegal NgoMIV site found at 1452
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None