Composite

Part:BBa_K3002125

Designed by: Marlene Schlosser   Group: iGEM19_TU_Kaiserslautern   (2019-10-21)
Revision as of 01:18, 22 October 2019 by MSchlosser (Talk | contribs)


Level 1 Wildtype-PETase + ARS + sp20-His

This composite part contains the PAR-promoter (BBa_K3002027) in combination with the RPL23-Terminator (BBa_K3002006), the SP20-His-tag (BBa_K3002018), the secretion signal ARS (BBa_K3002009) and the coding sequence of the Wildtype-PETase (BBa_K3002004) plus the MoClo connectors for positions B1-B2 (BBa_K3002302), B2-B3 (BBa_K3002303), B4-B5 (BBa_K3002304) and B5-B6 (BBa_K3002305).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1075
    Illegal PstI site found at 2048
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 249
    Illegal PstI site found at 1075
    Illegal PstI site found at 2048
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1075
    Illegal PstI site found at 2048
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1075
    Illegal PstI site found at 2048
    Illegal NgoMIV site found at 810
    Illegal NgoMIV site found at 837
    Illegal NgoMIV site found at 2539
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None