Device

Part:BBa_K3292001

Designed by: Abril Diosdado Hernandez, Gerardo David Diaz Renovado   Group: iGEM19_Tec-Monterrey   (2019-10-16)
Revision as of 01:16, 22 October 2019 by AbrilDH (Talk | contribs)


T7-LacO Promoter + strong RBS + sialidase + sfGFP + 6x his-tag + double terminator


This device uses Bba_K2406020, Bba_J34801 and Bba_0010 to regulate the expression of a super folding GFP (Bba_I746916). The main objective of creating this device is to facilitate the secretion and characterization of any protein intracellularly produced after adding its nucleotide sequence next to the sfGFP.


<Usage and Biology The characterization of this part included a Bradford assay to quantify the amount of protein present in the medium for each of the proposed constructs.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 721
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1986
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 646
    Illegal BsaI.rc site found at 510
    Illegal BsaI.rc site found at 1647
    Illegal SapI.rc site found at 2055


[edit]
Categories
Parameters
None