Measurement

Part:BBa_K3046009

Designed by: Philip Srensen, Marcus Medom Ryding   Group: iGEM19_DTU-Denmark   (2019-10-16)
Revision as of 01:06, 22 October 2019 by JMejlsted (Talk | contribs)


Fungal MoClo promoter test device

This is a device for testing promoters by expressing mCherry.


Usage and Biology

The bacterial promoter, BBa_K3046017, can be cut out using the Type IIs restriction enzyme as shown in the figure below. Following this, a promoter following the level 0 promoter + 5' UTR MoClo standard can be inserted and the assembled construct can be used for characterization of the promoter.

<figure> <img style="padding:28px;" src="https://2019.igem.org/wiki/images/9/96/T--DTU-Denmark--PromEx.svg" alt="This figure shows a bacterial promoter in front of the fluorescent protein mCherry being replaced by a synthetic promoter when cut with BsaI, thus allowing for expression in our organism." class="safetyfirstimg"> <figcaption>The figure shows the general strategy for replacing the prokaryotic promoter BBa_K3046017 upstream of mCherry with a synthetic promoter using Golden Gate assembly.</figcaption> </figure>

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1158
    Illegal BamHI site found at 1418
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 232
    Illegal BsaI.rc site found at 6


[edit]
Categories
//chassis/eukaryote
//classic/reporter/constitutive
Parameters
None