Composite
Part:BBa_K3044007
Designed by: Anne Sofie Løgstrup Henriksen Group: iGEM19_SDU-Denmark (2019-09-27)
oriT-R combined with FimH
oriT-R make the conjugation of the plasmid possible if a compatible conjugative plasmid is present. The FimH gene codes for an adhesion protein and it makes the expression of type 1 pili even more efficient. The 2019 SDU-Denmark team tested this construct to test if the conjugation between E. coli K12 Top10 would become more efficient. This was not the case, and it worsened the conjugation efficiency.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 117
Illegal NgoMIV site found at 127 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 19
[edit]
Categories
Parameters
None |