Composite

Part:BBa_K3044007

Designed by: Anne Sofie Løgstrup Henriksen   Group: iGEM19_SDU-Denmark   (2019-09-27)
Revision as of 01:03, 22 October 2019 by Annemette (Talk | contribs)

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oriT-R combined with FimH

oriT-R make the conjugation of the plasmid possible if a compatible conjugative plasmid is present. The FimH gene codes for an adhesion protein and it makes the expression of type 1 pili even more efficient. The 2019 SDU-Denmark team tested this construct to test if the conjugation between E. coli K12 Top10 would become more efficient. This was not the case, and it worsened the conjugation efficiency.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 117
    Illegal NgoMIV site found at 127
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 19


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Categories
Parameters
None