Part:BBa_K3042000

Dinoflagellate Codon Optimized RFP

This part is a codon optimized version of RFP for use in use in Dinoflagellates, a single celled organism. This part was created to be used in DinoIII, a dinoflagellate backbone vector . The sequence for this part was synthesized via Snapgene. Considering dinoflagellates have preference when it comes to specific amino acids, the favorable codons of the dinoflagellates were chosen using the chart below to ensure the successful translation of the red fluorescent protein. This part can be used as a reporter/indicator of a successful transformation. Positive results are demonstrated by red fluorescence emitted from the cells.

Figure 1. Codon Optimized Chart for Oxyrrhis marina

This was the chart we used to design the RFP part in Snapgene, to be edited into the Dino III plasmid in place of the existing GFP part BBa_K3042005

mRFP1- monomeric derivative of Dsred (Campbell et al., 2002)

Brittany N. Sprecher, Huan Zhang, Senjie Lin (2019, April 9). Nuclear gene transformation in a dinoflagellate. doi: 10.1101/602821<p> Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 13
  
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 696
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 13
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 13
  
• 1000
  COMPATIBLE WITH RFC[1000]