Composite

Part:BBa_K3002119

Designed by: Marlene Schlosser   Group: iGEM19_TU_Kaiserslautern   (2019-10-21)
Revision as of 00:50, 22 October 2019 by MSchlosser (Talk | contribs)


Level 1 Hygromycin resistance + Tub2 Promoter + Tub2 Terminator

This composite part contains the Tub2-promoter (BBa_K3002011) in combination with the Tub2-terminator (BBa_K3002012), and the coding sequence of the Hygromycin resistance (BBa_K3002013) plus the MoClo connectors for positions B2-B3 (BBa_K3002303) and B5-B6 (BBa_K3002305).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1408
    Illegal XhoI site found at 250
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 773
    Illegal NgoMIV site found at 955
    Illegal NgoMIV site found at 1326
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None