Plasmid

Part:BBa_K3201021

Designed by: Anastasios Galanis, Nikolaos Karvelas, Nikolas Kalavros, Fani Zoumpou, Iason Papadopoulos, Anna Maria Tsiatsiani, Alexandra Karvela   Group: iGEM19_Athens   (2019-10-21)
Revision as of 00:47, 22 October 2019 by AptamersBoi (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


pEKATI (Plasmid for Aptamer Directed Evolution using EvolvR)

This is the complete construct of the MEDEA project, bearing the EvolvR mechanism of directed evolution.

pEKATI contains the EvolvR system using nCas9-PolI3M-TBD as the mutagenesis system, first proposed by Halperin et al. (2018). The construct contains the Aptamer Binding Cassette for directed evolution of aptamers using this system.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 296
    Illegal EcoRI site found at 388
    Illegal EcoRI site found at 988
    Illegal XbaI site found at 2272
    Illegal XbaI site found at 3763
    Illegal XbaI site found at 12320
    Illegal XbaI site found at 13230
    Illegal SpeI site found at 15158
    Illegal PstI site found at 234
    Illegal PstI site found at 9932
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 296
    Illegal EcoRI site found at 388
    Illegal EcoRI site found at 988
    Illegal NheI site found at 14203
    Illegal NheI site found at 14220
    Illegal NheI site found at 14243
    Illegal NheI site found at 15135
    Illegal SpeI site found at 15158
    Illegal PstI site found at 234
    Illegal PstI site found at 9932
    Illegal NotI site found at 399
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 296
    Illegal EcoRI site found at 388
    Illegal EcoRI site found at 988
    Illegal BglII site found at 333
    Illegal BglII site found at 3787
    Illegal BglII site found at 12430
    Illegal BglII site found at 13251
    Illegal BamHI site found at 290
    Illegal BamHI site found at 7963
    Illegal BamHI site found at 10192
    Illegal BamHI site found at 12265
    Illegal XhoI site found at 284
    Illegal XhoI site found at 393
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 296
    Illegal EcoRI site found at 388
    Illegal EcoRI site found at 988
    Illegal XbaI site found at 2272
    Illegal XbaI site found at 3763
    Illegal XbaI site found at 12320
    Illegal XbaI site found at 13230
    Illegal SpeI site found at 15158
    Illegal PstI site found at 234
    Illegal PstI site found at 9932
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 296
    Illegal EcoRI site found at 388
    Illegal EcoRI site found at 988
    Illegal XbaI site found at 2272
    Illegal XbaI site found at 3763
    Illegal XbaI site found at 12320
    Illegal XbaI site found at 13230
    Illegal SpeI site found at 15158
    Illegal PstI site found at 234
    Illegal PstI site found at 9932
    Illegal NgoMIV site found at 4869
    Illegal NgoMIV site found at 8878
    Illegal NgoMIV site found at 13083
    Illegal AgeI site found at 8371
    Illegal AgeI site found at 10172
    Illegal AgeI site found at 12100
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 349
    Illegal BsaI.rc site found at 320
    Illegal SapI site found at 12082
    Illegal SapI.rc site found at 4950


[edit]
Categories
Parameters
None