Protein_Domain
SP-AntiNIP
Part:BBa_K157026
Designed by: iGEM Team Freiburg 2008 Group: iGEM08_Freiburg (2008-10-26)
SP-AntiNIP-GGGSlinker-Tm-Fluolinker-cYFP
A composite part consisting of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker (extracellular part), the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split fluorophor YFP with a fluolinker joined to its N-Terminus (intracellular part).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 868
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4
Illegal AgeI site found at 1255 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 12
[edit]
Categories
Parameters
//function/reporter/fluorescence
//proteindomain/binding
//proteindomain/internal
//proteindomain/binding
//proteindomain/internal
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