Regulatory
Part:BBa_K3189001
Designed by: Jehoshua Sharma, Nathanael Wilms, Connor Gianetto-Hill Group: iGEM19_Guelph (2019-10-16)
Phage Lambda-TetO
A modified phage lambda PL promoter with tet operator sites. This part functions as a promoter that allows for tetracycline-based transcriptional activation
The flasks in Figure 1 shows 50 mL E.coli cells transformed with gfp under the control of BBa_K3189001. On the plasmid containing BBa_K3189001 is the repressor, BBa_K3189004, needed for proper function of the promoter. Tetracycline was used to induce the system, resulting in GFP production and fluorescence is seen using a UV wand.
Figure 1: E. coli cells expressing GFP under the control of BBa_K3189001 induced with tetracycline.
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Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
| None |