Part:BBa_K3046033

IS2 downstream sequence for A. niger genome integration

This part is a long sequence of DNA that are homologous to a part of the albA conidial pigment gene. This homology can be used for homologous recombination of genomic constructs flanked by this IS2 sequence (along with the upstream sequence BBa_K3046032).

Characterization

We have demonstrated that the IS2 up- and downstream sequences work as expected qualitatively speaking. We did this by transforming a GFP reporter into Aspergillus niger ATCC1015, and as shown in figure 1, we were able to make the transformants express GFP as opposed to the wildtype control.

[[File:T--DTU-Denmark--GFPfung2.png]200px|fig.1|left|The two upper pictures show Aspergillus niger ATCC1015 transformed with the pPEA2P1 plasmid. The two pictures in the bottom show the same fungi, transformed with a self replicating AMA1 plasmid containing the PyrG marker]]

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 118
  Illegal PstI site found at 1262
  Illegal PstI site found at 1863
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 118
  Illegal PstI site found at 1262
  Illegal PstI site found at 1863
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 118
  Illegal BglII site found at 1360
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 118
  Illegal PstI site found at 1262
  Illegal PstI site found at 1863
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 118
  Illegal PstI site found at 1262
  Illegal PstI site found at 1863
  Illegal NgoMIV site found at 1250
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 1751
  Illegal SapI site found at 2060