Composite

Part:BBa_K3002103

Designed by: Dorothée Klein   Group: iGEM19_TU_Kaiserslautern   (2019-10-21)
Revision as of 23:48, 21 October 2019 by MSchlosser (Talk | contribs)


Level 1 – Wildtype PETase + His (Phytobrick)

This composite part contains the PAR-promoter (BBa_K3002027) in combination with the RPL23-Terminator (BBa_K3002006), a HA-tag (BBa_K3002017) and the coding sequence of the wildtype PETase (BBa_K3002004) plus the MoClo connectors for positions B2-B3 (BBa_K3002303), B4-B5 (BBa_K3002304) and B5-B6 (BBa_K3002305).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1002
    Illegal PstI site found at 1975
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 249
    Illegal PstI site found at 1002
    Illegal PstI site found at 1975
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1002
    Illegal PstI site found at 1975
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1002
    Illegal PstI site found at 1975
    Illegal NgoMIV site found at 737
    Illegal NgoMIV site found at 764
    Illegal NgoMIV site found at 2415
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None