Coding

Part:BBa_K3208010:Design

Designed by: Slavil Peykov   Group: iGEM19_Bulgaria   (2019-10-21)
Revision as of 23:45, 21 October 2019 by Slavil (Talk | contribs) (→‎Design Notes)

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Red expression cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 787
    Illegal AgeI site found at 899
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1036
    Illegal BsaI.rc site found at 1


Design Notes

Use PCR to amplify this part with the corresponding overhangs for IIs restriction cloning.


Primers:

F: caatacgcaaaccgcctctc R: actctagtagagagcgttcacc


NB!!! Do not forget to add the Eco31I site in the 5' end of the primers (GAGACC) in the correct orientation for the enzyme to cut outside of the cassette.

Source

This part was is a modified version of part BBa_J04450 that is used as a red coding device in pSB1C3.

References