Coding

Part:BBa_K2996500

Designed by: Shifeng Jiang   Group: iGEM19_SJTU-BioX-Shanghai   (2019-10-03)
Revision as of 23:42, 21 October 2019 by Jsf19981231 (Talk | contribs)

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Zinc finger with flag tag

It is a sequence encoding protein that can recruit zinc ions for functioning.The protein can bind specific DNA sequence 5'-aTTAGGAGTGa-3'.To better purify the protein,we also added a flag tag at the C terminal of it. It is composed of mammalian synthetic transcription factors (SynTFs) based on Cys2-His2 zinc finger 21-16.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 304
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None