Coding

Part:BBa_K3117012

Designed by: Lena Schorr   Group: iGEM19_FAU_Erlangen   (2019-10-13)
Revision as of 23:16, 21 October 2019 by Lena Schorr (Talk | contribs)


pelB signal sequence

pelB is a signal sequence, which can target recombinant proteins to the periplasmic space. It uses the post-translational translocation pathway (Singh et al. 2013).


Usage and Biology

The secretion of the recombinant protein into the periplasma has distinct advantages like the oxidising environment for the formation of disulfide bonds, which does not occur in the reducing environment of the cytoplasm or foldases inside the periplasm which catalyse the formation and isomerisation of disulfide bonds (Schlegel et al. 2013).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 54
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None