Coding

Part:BBa_K3317001

Designed by: Erick Hernandez, Abigail Torres and Shakti Delgadillo   Group: iGEM19_UANL   (2019-10-15)
Revision as of 23:10, 21 October 2019 by Erick419 (Talk | contribs)

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Vanillate O-demethylase (LigM gene) from Sphingobium sp. SYK-6 with the pduP signal peptide

Vanillate O-demethylase (LigM)(BBa_K3317000), was modified with a signal peptide at its C-terminal to be introduced into bacterial microcompatments (BMCs) This signal peptide corresponds to the first 19 amino acids of the psuP enzyme together with a flexible linker G42 (Wagner, H. J et. al 2017) This dimeric enzyme is tetrahydrofolate-dependent.


Wild type enzyme information: E. C. 2.1.1.341

Km 0.078

Kcat 7.1292

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 369
    Illegal NgoMIV site found at 1150
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1372


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Parameters
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