Coding

Part:BBa_K3117020

Designed by: Lena Schorr   Group: iGEM19_FAU_Erlangen   (2019-10-13)
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scFv VH against CD3 codon optimized for CHO expression

Part BBa_K3117020 is a single chain variable fragment (scFv) of the anti-CD3 antibody. This fragment was used to generate a fusion protein to create a bispecific antibody on the one hand directed against CD3, on the other part against GPA33.

Usage and Biology

CD3 is co-expressed with the T cell receptor on T cells. Binding leads to downstream signaling cascades and activation of the T cell (Chetty et al., 1994).

References

1. Chetty, R.; Gatter, K. (1994): CD3: structure, function, and role of immunostaining in clinical practice. In: The Journal of pathology 173 (4), S. 303–307. DOI: 10.1002/path.1711730404.

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