Coding

Part:BBa_K3317001

Designed by: Erick Hernandez, Abigail Torres and Shakti Delgadillo   Group: iGEM19_UANL   (2019-10-15)
Revision as of 22:46, 21 October 2019 by Erick419 (Talk | contribs)


Vanillate O-demethylase (LigM gene) from Sphingobium sp. SYK-6 with the pduP signal peptide

Vanillate O-demethylase (LigM)(BBa_K3317000), was modified with a signal peptide at its C-terminal to be introduced into bacterial microcompatments (BMCs) This signal peptide corresponds to the first 19 amino acids of the psuP enzyme together with a flexible linker G42 (Wagner, H. J et al. 2017)

This dimeric enzyme is tetrahydrofolate-dependent.

File:T--UANL--LigM.jpeg
alt text

Wild type enzyme information: E. C. 2.1.1.341

Km 0.078

Kcat 7.1292

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 369
    Illegal NgoMIV site found at 1150
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1372


[edit]
Categories
Parameters
None