Protein_Domain
CEA

Part:BBa_K3284002:Design

Designed by: Bella Stevanny   Group: iGEM19_SriwijayaGold   (2019-10-21)
Revision as of 22:34, 21 October 2019 by Bellastevanny (Talk | contribs)


Carcinoembryonic antigen (CEA) with His tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 40
    Illegal NotI site found at 354
    Illegal NotI site found at 2137
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 532
    Illegal AgeI site found at 1618
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Tumor markers are molecules occurring in blood or tissue that are produced by a tumor associated with a cancer or by the host in response to the cancer. Measurement or identification of tumor markers is useful for clinical diagnosis or patient management. Carcinoembryonic antigen (CEA), the first oncofetal antigens, consists of a large family of cell surface glycoprotein (molecular weight: 150–300 kDa). It is associated with plasma membrane of tumor cells, from which it may be released into the blood. In lung cancer serological tests, one of the most commonly used circulating markers is CEA and CYFRA. But our team chose CEA as our main target to be diagnosed, because CEA is proven to have a high sensitivity and specificity other than tumor markers.

In this project, CEAker is a synthetically combined receptor used as the diagnostic tool system. TGFBR1, which is originally found in human body, directly interacts with Carcinoembryonic Antigen (CEA). CEA itself functions as an intercellular adhesion molecule and is upregulated in a wide variety of human cancers, including lung cancer. CEA-binding domain mainly lodged in TGFBR1’s extracellular environment.

Figure 1. CEA structure.

We added His tag in order to synthesize pure CEA (our receptor ligand).




Source

CEACAM5.

References