Coding

Part:BBa_K3117020:Design

Designed by: Lena Schorr   Group: iGEM19_FAU_Erlangen   (2019-10-13)
Revision as of 22:31, 21 October 2019 by Lena Schorr (Talk | contribs)


scFv VH against CD3 codon optimized for CHO expression


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 142
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was codon optimized for expression in mammalian cells.


Source

The sequence of this part was provided by Prof. Matthias Peipp from the Christian-Albrechts-Universität of Kiel.

References

[1]: Carreras-Sangrà, Nelson; Tomé-Amat, Jaime; García-Ortega, Lucía; Batt, Carl A.; Oñaderra, Mercedes; Martínez-del-Pozo, Alvaro et al. (2012): Production and characterization of a colon cancer-specific immunotoxin based on the fungal ribotoxin α-sarcin. In: Protein engineering, design & selection : PEDS 25 (8), S. 425–435. DOI: 10.1093/protein/gzs032.

[2]: Chetty, R.; Gatter, K. (1994): CD3: structure, function, and role of immunostaining in clinical practice. In: The Journal of pathology 173 (4), S. 303–307. DOI: 10.1002/path.1711730404.