Regulatory

Part:BBa_K2916059:Design

Designed by: Konstantinos Ragios   Group: iGEM19_EPFL   (2019-09-29)
Revision as of 22:25, 21 October 2019 by Konstantinos (Talk | contribs)


Toehold for Bois Noir 2.1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 96
  • 1000
    COMPATIBLE WITH RFC[1000]


Source

The sequenced was designed by the 2019 EPFL team and was ordered as a primer overhand, for ampliying sfGFP, and manufacted by IDT.


References

Green, Alexander A., et al. “Toehold Switches: De-Novo-Designed Regulators of Gene Expression.” Cell, vol. 159, no. 4, Nov. 2014, pp. 925–39. DOI.org (Crossref), doi:10.1016/j.cell.2014.10.002.