Part:BBa_K3183005

AgrC2 Quorum Sensing Histidine Kinase; C. difficile

Quorum Sensing membrane-bound histidine kinase, shown previously to dimerize and phosphorylate AgrA2 in C. difficile. It is one of the members of the two-component detection system, adapted from C. difficile, that can bind the Autoinducing peptide (AIP), and, in response, transduce signalling to AgrA2.¹ This gene is located in C. difficile CD196 genome.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Use by Team Oxford 2019

This membrane protein is part of our detection system in L. reuteri. The composite parts containing this AgrC2 protein are BBa_K3183102, and BBa_K3183300.

Reference

1. Darkoh, Charles, et al. “Toxin Synthesis by Clostridium Difficile Is Regulated through Quorum Signaling.” MBio, vol. 6, no. 2, May 2015, pp. e02569-14. mbio.asm.org, doi:10.1128/mBio.02569-14.