Part:BBa_K3183005

AgrC2 Quorum Sensing Histidine Kinase; C. difficile

Quorum Sensing membrane-bound histidine kinase, shown previously to dimerize and phosphorylate AgrA2 in C. difficile. It is one of the members of the two-component detection system, adapted from C. difficile, that can bind the Autoinducing peptide (AIP), and, in response, transduce signalling to AgrA2. This gene is located in C. difficile CD196 genome.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Use by Team Oxford 2019

This membrane protein is part of our detection system in L. reuteri. The composite parts containing this AgrC2 protein are BBa_K3183102, and BBa_K3183300.

Reference

1. Darkoh, Charles, et al. “Toxin Synthesis by Clostridium Difficile Is Regulated through Quorum Signaling.” MBio, vol. 6, no. 2, May 2015, pp. e02569-14. mbio.asm.org, doi:10.1128/mBio.02569-14.