Coding

Part:BBa_K3117036:Design

Designed by: Lena Schorr   Group: iGEM19_FAU_Erlangen   (2019-10-18)
Revision as of 22:21, 21 October 2019 by Lena Schorr (Talk | contribs)


scFv VL sequence of anti-GPA33 antibody codon optimized for expression in E.coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was codon optimized for expression in E.coli.


Source

The sequence of this part was provided by Prof. Matthias Peipp from the Christian-Albrechts-Universität of Kiel.

References

[1]: Carreras-Sangrà, Nelson; Tomé-Amat, Jaime; García-Ortega, Lucía; Batt, Carl A.; Oñaderra, Mercedes; Martínez-del-Pozo, Alvaro et al. (2012): Production and characterization of a colon cancer-specific immunotoxin based on the fungal ribotoxin α-sarcin. In: Protein engineering, design & selection : PEDS 25 (8), S. 425–435. DOI: 10.1093/protein/gzs032.