Coding

Part:BBa_K3166004:Experience

Designed by: Yijia Ren   Group: iGEM19_SEFLS_Shanghai   (2019-09-11)
Revision as of 22:19, 21 October 2019 by Jimmychen (Talk | contribs) (→‎Applications of BBa_K3166004)

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Applications of BBa_K3166004

It was reported that the dehydrosqualene desaturase CrtN from Staphylococcus aureus converts squalene into yellow carotenoid pigments, permitting the colorimetric detection of squalene produced in the cell. Sub-cloning CrtN into the plasmid helps us to screen out squalene synthase with high activity rapidly.

T--SEFLS_Shanghai--BBa_K3166004-fig1.png

It was sub-cloned into genes below:

pETDuet-1T7-KSS-2T7-CrtN(pET-KN) BBa_K3166997

pETDuet-1T7-NSS-2T7-CrtN(pET-NN) BBa_K3166800

pETDuet-1T7-YSS-2T7-CrtN(pET-YN) BBa_K3166998

pETDuet-1T7-thSQS-2T7-CrtN(pET-thN) BBa_K3166998

pETDuet-rbs-idi-rbs-ispA-rbs-YSS-2T7-CrtN（best composite part BBa_K3166808https://parts.igem.org/Part:BBa_K3166058）

User Reviews

UNIQ1c8587b99bc0865a-partinfo-00000000-QINU UNIQ1c8587b99bc0865a-partinfo-00000001-QINU