Coding

Part:BBa_K3192018

Designed by: Simonne Guenette   Group: iGEM19_Virginia   (2019-10-12)
Revision as of 22:17, 21 October 2019 by Simonneguenette (Talk | contribs)


styD

This part contains the gene styD which encodes for the protein phenylacetaldehyde dehydrogenase. This gene was obtained from Pseudomonas putida S12, and combines in conjunction with the overall cluster styABCDE. This is the final step in the degradation of styrene to phenylacetic acid. This catalyzes the oxidation of phenylacetaldehyde to phenylacetic acid.1

References:

1) Crabo, A. G. et al.Structure and biochemistry of phenylacetaldehyde dehydrogenase from the Pseudomonas putida S12 styrene catabolic pathway. Archives of Biochemistry and Biophysics 616,47–58 (2017).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1380
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 540
    Illegal NgoMIV site found at 907
    Illegal AgeI site found at 730
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1318


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Categories
Parameters
None