Coding
AgrA2

Part:BBa_K3183004:Design

Designed by: David Schramm   Group: iGEM19_Oxford   (2019-10-16)
Revision as of 22:15, 21 October 2019 by Mrfelf (Talk | contribs) (→‎References)


AgrA2 Quorum Sensing Transcription Factor; C. difficile


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized for L. reuteri using [http://www.kazusa.or.jp/codon/cgi-bin/showcodon.cgi?species=1598 kazusa codon-usage tables.]
RF10, 12, 21, 23, 25, and 1000 Compatible

Source

Derived from the C. difficile strain CD196 Genome; synthesized in IDT gBlocks.

References

1. Martin, Melissa J., et al. “The Agr Locus Regulates Virulence and Colonization Genes in Clostridium Difficile 027.” Journal of Bacteriology, vol. 195, no. 16, Aug. 2013, pp. 3672–81. PubMed, doi:10.1128/JB.00473-13.