Coding

Part:BBa_K3137005:Design

Designed by: Jiamin Chen   Group: iGEM19_JiangnanU_China   (2019-10-07)
Revision as of 22:14, 21 October 2019 by LWJ (Talk | contribs) (→‎References)

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gntR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 425
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The results of overexpression of a single gene against phage are not satisfactory. It is supposed that it requires multiple genes to interact with each other in order to function properly, which may be our future work.

Source

We have screened the mutant strains and performed the genome analysis of the mutant strains we obtained, after that, we found the mutant protein gntR which is associated with the phage resistance.

References

Hoskisson P A , Sébastien Rigali. Chapter 1: Variation in form and function the helix-turn-helix regulators of the GntR superfamily[J]. Advances in applied microbiology, 2009, 69(69):1-22.