Regulatory

Part:BBa_K135000:Design

Designed by: Oliver Purcell   Group: iGEM08_BCCS-Bristol   (2008-10-20)
Revision as of 20:59, 26 October 2008 by Mari (Talk | contribs) (→‎References)

pCpxR (CpxR responsive promoter)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

This part comes from the genome of E.coli TR235, as used in the PNAS paper of Otto and Silhavy mentioned on the main page. TR235 is K-12 MC4100 containing a pCpxR-LacZ fusion inserted at the (Lambda)att site, plus some other modifications (see Otto and Silhavy). The part is a portion of the CpxR responsive region used in these LacZ fusions.

References

Karen Otto and Thomas J. Silhavy (2002): Surface sensing and adhesion of Escherichia coli controlled by the Cpx-signaling pathway, PNAS, Vol. 99., No, 4., 2287-2292.