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Part:BBa_K3037004

Designed by: Arnau Pérez Roig   Group: iGEM19_TU_Dresden   (2019-10-11)
Revision as of 22:12, 21 October 2019 by Psantos (Talk | contribs) (→‎Characteristics)

Linker

Linker
Function Linker for fusion proteins
Use in Escherichia coli
RFC standard Freiburg RFC25 standard
Backbone pSB1C3
Submitted by Team: TU_Dresden 2019


Overview

The TU Dresden team 2019 used this linker in order to make their fusion protein BBa_K3037003 (more information).

This BioBrick was used to make the fusion proteins (BBa_K3037005) and (BBa_K3037003) as well.

Characterization

This part, encodes for a linker used in between translationally fused proteins. The amino acid composition is "GSAGSAAGSG". The high amounts of glycine and serine residues make it a very flexible linker.

Sequence

This BioBrick is flanked by the RFC25 standard.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None