Part:BBa_K3267000
Bioelectric Relay Construct
This part will respond to the electric potential via various redox modulators and the transformed cell begins to have the activated transcription factor, which is SoxR protein. This transcription factor protein binds to the bidirectional promoter region, then cells translate more SoxR and LuxI.
Biology
The Relay part contains E.coli. intrinsic the bidirectional promoter region of the SoxRS regulon. The SoxR coding sequence is truncated and LuxI coding region is added followed by a ssRA tag. LuxI gene is originally from the bacterium A. fischeri and encodes Acyl-homoserine-lactose synthase. LuxI synthases Acyl-homoserine-lactose (AHL) which is known as autoinducing peptides. The peptides are released in the extracellular environment, and other cells are able to detect the change in concentration of this peptide. This autoinduction process is called quorum-sensing (QS).
Usage
In our device, we use multiple redox modulators to convert electric stimuli into chemical signals. Ferrocyanide/Ferricyanide in the extracellular environment. The redox-active molecule, Pyocyanin, initiates the expression of this part by activating the intrinsic SoxR transcription factor. Under the positive electric potential, reduced Ferrocyanide gets oxidized to become Ferricyanide, and the signal is transferred to the intracellular environment via Menaquinone (MQ) is oxidized by Ferricyanide through the membrane. Then, the reduced Pyocyanin that initiated the reaction once again oxidized to activate SoxR protein. This positive feedback loop creates LuxI protein, which synthases the quorum sensing hormone, acyl-homoserine lactone (AHL).
Characterization
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1165
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 49
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