Signalling

Part:BBa_K3267000

Designed by: Yuma Kanazawa   Group: iGEM19_NYU_Shanghai   (2019-10-15)
Revision as of 21:53, 21 October 2019 by Yumakk (Talk | contribs) (→‎Biology)


Bioelectric Relay Construct

This part will respond to the electric potential via various redox modulators and the transformed cell begins to have the activated transcription factor, which is SoxR protein. This transcription factor protein binds to the bidirectional promoter region, then cells translate more SoxR and LuxI.

Biology

The Relay part contains E.coli. intrinsic the bidirectional promoter region of the SoxRS regulon. The SoxR coding sequence is truncated and LuxI coding region is added followed by a ssRA tag. LuxI gene is originally from the bacterium A. fischeri and encodes Acyl-homoserine-lactose synthase. LuxI synthases Acyl-homoserine-lactose (AHL) which is known as autoinducing peptides. The peptides are released in the extracellular environment, and other cells are able to detect the change in concentration of this peptide. This autoinduction process is called quorum-sensing (QS).

Usage

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1165
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 49


[edit]
Categories
Parameters
None