Composite

Part:BBa_K3117046:Design

Designed by: Lena Schorr   Group: iGEM19_FAU_Erlangen   (2019-10-18)
Revision as of 21:32, 21 October 2019 by DSchreyer (Talk | contribs)


scFv bispecific antibody against GPA33 and CD3 codon optimized for E.coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 54
    Illegal AgeI site found at 519
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Figure 1: Modelling of the 4x[GGGS] linker



The part consists of humanized anti-CD3 and anti-GPA33 single-chain variable fragments (scFv) (BBa_K3117020, BBa_K3117021, BBa_K3117022, BBa_K3117023), which are connected by a flexible [GGGGS]4 linker (BBa_K3117004). The scFvs are formed by the variable regions of the heavy and light chain of the respective antibodies that are connected by either a [GGGGS]4 or [GGGGS]3 linker (BBa_K3117004, BBa_K3117028). The sequence contains a C-terminal His-Tag (BBa_K3117005) for easy purification and detection. Secretion of the protein is ensured by an Igk leader (BBa_K3117006). When the protein passes the membrane, this leader domain is cleaved off.