Part:BBa_K3257033

mCherry

This part is a red fluorescence protein mCherry. We added loxP sites at both ends of it to test if Cre recombinase worked properly.

Figure 1. The relative fluorescence intensity of mCherry and its mutants mCherry represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding red fluorescence protein mCherry. The mCherry_Q158X and the mCherry_D159X represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding nonsense mutated red fluorescence protein mCherry. Significant difference between them shows that mCherry can be expressed and function well inside E.coli cells.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]