Generator

Part:BBa_K2971000:Design

Designed by: Simen Hermansen   Group: iGEM19_UiOslo_Norway   (2019-10-15)
Revision as of 21:16, 21 October 2019 by Simen (Talk | contribs) (→‎Design Notes)

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crtE from Deinococcus radiodurans


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 841
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 43


Design Notes

Codon optimzed for expression E.coli and to make it biobrick compatible.

The gene was selected from Deinococcus radiodurans due to the organism’s extremophilic properties. D.radiodurans exhibit several traits that would make it ideal in our imagined biogenic solar cell (see our design page), such as a high resistance towards ionizing radiation and dehydration. Although the translated protein does not necessarily exhibit the same resistances as the whole organism, it was selected to highlight our interest in D.radiodurans. The gene was placed in an expression vector under pBAD induction so that we could overexpress the protein to test its enzymatic activity. Due to time restraints, and the general difficulty of characterizing carotenogenic enzymes, this was not achieved.

Source

Deinococcus radiodurans.

References