Tag

Part:BBa_K3117005

Designed by: Lena Schorr   Group: iGEM19_FAU_Erlangen   (2019-10-13)
Revision as of 20:59, 21 October 2019 by DSchreyer (Talk | contribs)


6xHis-Tag

This part contains six times Histidin (His), which can be used for protein purification. It has the characteristic, that it can bind to Ni2+ ions and form a chelat-complex. Therefore, the 6xHis tag can be used to extract the protein using Nickel-based affinity columns. Furthermore, established anti-His antibodies can bind to the 6xHis-tag. This can be used for FACS measurements or protein detection.


[edit]
Categories
Parameters
None