Composite

Part:BBa_K3110009:Design

Designed by: Mrugank Dake   Group: iGEM19_IISER_Tirupati   (2019-10-14)
Revision as of 20:56, 21 October 2019 by Dibyasaha (Talk | contribs) (→‎Source)


Medium Promoter Weak RBS lldD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1201
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 638


Design Notes

lldD was codon optimized to meet synthesis requirements and to remove the illegal EcoRI site present in the genomic lldD.



Source

The lldD Synthesized from Twist Bioscience. Specific Promoter and RBS with D flank and Terminator with D flank was ordered from IDT and all the 3 parts were merged together using SOEing (Spliced Extension Overlapped) PCR.

References

Aguilera, L., Campos, E., Gimenez, R., Badia, J., Aguilar, J., & Baldoma, L. (2008). Dual Role of LldR in Regulation of the lldPRD Operon, Involved in L-Lactate Metabolism in Escherichia coli. Journal of Bacteriology, 190(8), 2997–3005. doi:10.1128/jb.02013-07