Regulatory

Part:BBa_K3257003

Designed by: Yi Qiao   Group: iGEM19_Fudan-TSI   (2019-09-03)
Revision as of 20:50, 21 October 2019 by YY12138 (Talk | contribs)

lacIq Promoter

This part is lacIq promoter and it can be used to initiate the expression of lacI protein. In the lacIq allele, a single base change in the promoter boosts expression of the lacI gene about 10-fold.

Biology

Figure 1. The relative fluorescence intensity of EGFP with lacIq promoter measured by 96-well plate reader EGFP represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding EGFP protein. The Negative represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding nonsense mutated EGFP protein. Significant difference between them shows that EGFP can be expressed and function well inside E.coli cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None