Regulatory

Part:BBa_K3257000

Designed by: Yi Qiao   Group: iGEM19_Fudan-TSI   (2019-09-03)
Revision as of 20:41, 21 October 2019 by YY12138 (Talk | contribs)


T7 Promoter

Promoter for bacteriophage T7 RNA polymerase.

Biology

Figure 2. The relative fluorescence intensity of EGFP with T7 promoter measured by 96-well plate reader EGFP represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding EGFP protein. The Negative represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding nonsense mutated EGFP protein. Significant difference between them shows that EGFP can be expressed and function well inside E.coli cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None