Coding

Part:BBa_K3110049:Design

Designed by: Swapnil Bhagat   Group: iGEM19_IISER_Tirupati   (2019-10-16)
Revision as of 20:35, 21 October 2019 by Dibyasaha (Talk | contribs) (→‎References)

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YebF


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We have taken the sequence which iGEM team of Oxford-2015 used (Part:BBa_K1659002) and optimized it using Twist Bioscience codon optimized tool to make it synthesize compatible.

Source

We ordered the sequence from Twist Bioscience along with IL12-FLAG

References

  • parts.igem.org/Part:BBa_K1659002
  • Zhang, G., Brokx, S., & Weiner, J. H. (2005). Extracellular accumulation of recombinant proteins fused to the carrier protein YebF in Escherichia coli. Nature Biotechnology, 24(1), 100–104. doi:10.1038/nbt1174