RBS

Part:BBa_K3192001

Designed by: Simonne Guenette   Group: iGEM19_Virginia   (2019-10-12)
Revision as of 20:26, 21 October 2019 by Simonneguenette (Talk | contribs)


Custom RBS Optimzed for BBa_K3192021

Synthetic Ribosomal Binding site for BBa_K3192021, a gene that encodes for the phaB gene.


The 2019 Virginia iGEM Team sought efficiency and productivity in designing their genetic construct as the device required two plasmids to operate. Two plasmids bears a large metabolic load on the chassis, so, synthetic ribosomal binding sites were developed in order to optimize translations rates for the genes used in the styrene-degrading and polyhydroxybutyrate-producing plasmids. This ribosomal binding site is optimized for part BBa_K3192015 with a translation initiation rate of 176782.37.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None