Coding

Part:BBa_K3110048:Design

Designed by: Amartya Pal   Group: iGEM19_IISER_Tirupati   (2019-10-15)
Revision as of 20:12, 21 October 2019 by Dibyasaha (Talk | contribs) (→‎Design Notes)


IL12 FLAG-tag (Codon Optimized)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimisation was done using Twist Codon optimisation too to make it synthesis compatible in E.coli. The FLAG-tag at the end can be used to detect IL12 produced by E.coli. Also took help from the sequence designed by UNICAMP EMSE Brazil team (iGEM2011) - Part:BBa_K554005

Source

Sequence was synthesized by Twist Bioscience.

References

  • K. R. Morris et al, Expression of biologically active recombinant porcine interleukin-12 from Escherichia coli, Veterinary Immunology and Immunopathology, 2008, 126 Link to PubMed
  • Sperandio V, Torres AG, Kaper JB (2002) Quorum sensing Escherichia coli regulators B and C (QseBC): a novel two-component regulatory system involved in the regulation of flagella and motility by quorum sensing in E. coli. Mol Microbiol 43: 809–821
  • Part:BBa_K554005 designed by UNICAMP EMSE Brazil team