Regulatory

Part:BBa_K3046010:Design

Designed by: Marcus Medom Ryding, Mikkel Rasmus Hansen   Group: iGEM19_DTU-Denmark   (2019-10-16)
Revision as of 20:12, 21 October 2019 by JMejlsted (Talk | contribs)

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PLEAPglaA_1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 342
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 354
    Illegal BamHI site found at 165
    Illegal XhoI site found at 599
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is optimised to be compatible with the MoClo standard, Mobius assembly standard and not to contain any SwaI cut sites.

Source

This part is created on the basis of the upstream region of the glaA ORF (Systematic ORF name: An03g06550) of many aspergillus genomes. It has been created using the proHMMoter software with added noise, as a part of the LEAP project, described in further detail here: https://2019.igem.org/Team:DTU-Denmark/Model

References

[1] P. Schape et al., “Updating genome annotation for the microbial cell factory Aspergillus niger using gene co-expression networks.,” Nucleic Acids Res., vol. 47, no. 2, pp. 559–569, Jan. 2019.